B5 medium has been defined for the growth of cell suspensions of soybean root cells in the presence of 2,4 D. Nitrate was required in a concentration of 20-30 mM. An addition of 2 mM ammonium sulphate led to an increase in cell growth. NH4+ when added as the sole source of nitrogen, did not support growth. Similar results were obtained when NH4NO3 was substituted for (NH4)2SO4. However, ammonium ions depressed growth when the concentration exceeded 2 mM. Variations in the concentrations of phosphate, calcium and magnesium resulted in relatively minor changes in growth rate. Thiamine is known to be an essential nutrient for cell growth and is increased in concentration up to 10 mg/l. B5 medium has been defined for the growth of cell suspensions of soybean root cells in the presence of 2,4 D. Nitrate was required in a concentration of 20-30 mM. An addition of 2 mM ammonium sulphate led to an increase in cell growth. NH4+ when added as the sole source of nitrogen, did not support growth. Similar results were obtained when NH4NO3 was substituted for (NH4)2SO4. However, ammonium ions depressed growth when the concentration exceeded 2 mM. Variations in the concentrations of phosphate, calcium and magnesium resulted in relatively minor changes in growth rate. Thiamine is known to be an essential nutrient for cell growth and is increased in concentration up to 10 mg/l.
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